FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30089885
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG169450.014.119022145
TACGTCC1190800.014.003005145
GTACGTC1310550.013.757973145
CGTCCTG1083850.013.658997145
ACGTCCT1124100.013.466596145
GGTGTAG589400.012.964708145
TGTACGT1455700.012.814456145
GTGTACG1603050.012.522993145
AGTGTAC1815750.011.954407145
GTGTAGT247400.011.692448145
GAGTGTA2020050.011.635448145
TGGTGTA696600.011.614834145
CTGGTGT928400.010.963879145
AGAGTGT2343850.010.297132145
ATCGGAC72900.09.944999145
AAGAGTG2543650.09.893034145
AAAGAGT2741050.09.773042145
GTCCTGG1354000.09.750424145
TCCTGGT1167250.09.534036145
ACGTCCG44550.09.275971145
Produced by FastQC (version 0.11.5)