FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44394426
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC65050.021.72894344-45
TATGCCG65150.021.28007146-47
TCGTATG65800.021.19538142-43
CTCGTAT67150.019.68351742-43
GATCGGA151050.019.295991
ATCGGAA161450.018.1315822
TCTCGTA86300.015.56104340-41
TCGGAAG199300.014.8295873
TCACGAT97500.014.74456734-35
ATCTCGT101550.013.50212240-41
ATGCCGT106550.013.14402246-47
CGGAAGA240550.012.5188664
CGTCTGA122950.012.34325216-17
ACGATCT111550.012.30331236-37
CACGATC119200.011.90801736-37
ATCACGA130100.011.41103534-35
ACACGTC129750.011.3502612-13
CATCACG139100.010.67340132-33
GCCGTCT130450.010.62536348-49
TGCCGTC136700.010.50046748-49
Produced by FastQC (version 0.11.5)