FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32331050
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC187450.018.796915145
CTCGTAT213850.018.035912145
TCGTATG185750.017.329653145
CGATAGA655000.015.728542145
GCGATAG725200.015.425664145
AGCGATA819200.015.390239145
TATGCCG146050.015.388486145
ATCTCGT318250.015.376982145
CGCGTAT40850.015.0856145
TCTCGTA261650.014.93495145
CAGCGAT989950.014.420096145
ATCGCGT57800.014.048368145
TCGCGTA49550.013.753713145
ACAGCGA1119900.013.614314145
GATCTCG420900.012.780878145
CACAGCG1300250.012.350445145
GATAGAT846000.011.749052145
GTATGCC242850.011.73249145
ATGCCGT184500.011.670671145
AGATCTC622100.010.7799635145
Produced by FastQC (version 0.11.5)