FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32331050
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG158400.013.685494145
TAGGCTA864700.013.390102145
GCTATAG595300.012.970544145
AGGCTAT858350.012.754311145
GTAGGCT1051700.012.684415145
GGCTATA679550.012.333345145
ATAGTGT440550.012.178142145
CTATAGT532900.011.9588145
AGTGTAG1644200.011.940939145
TATAGTG495600.011.84945145
TGTAGGC1338900.011.728832145
GAGTGTA1698850.011.565349145
TAGGCGA160050.011.324755145
AGGCGAT181400.011.150938145
AGAGTGT2007850.010.536551145
AAGAGTG2204650.010.233976145
CGATAGT84350.010.142415145
GCGATAG102500.010.044051145
GTGTAGG4935850.09.9633255145
AAAGAGT2387100.09.952915145
Produced by FastQC (version 0.11.5)