FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32805678
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT202950.018.612057145
GAAATCG188950.018.110931145
TCGTATG171700.017.692509145
CGTATGC183200.016.858925145
CGCGAAA641800.016.28963145
GCGAAAT593000.015.967401145
TATGCCG148900.015.922042145
CGCGTAT54800.015.876193145
CGAAATC552850.015.645118145
AATCTCG345750.015.601144145
CCGCGAA743200.015.559681145
ATCGCGT74900.015.100401145
TCTCGTA256600.015.087953145
AATCGCG78650.015.025696145
TCCGCGA842250.014.926355145
ATCTCGT306500.014.571221145
TCGCGTA65200.014.344579145
ACTCCGC1067650.013.8870535145
CTCCGCG1001700.013.599872145
CACTCCG1207900.013.132941145
Produced by FastQC (version 0.11.5)