FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28955839
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG369900.011.760981118-119
GTCGTGT341650.011.706429118-119
TCGTGTA300200.011.688395118-119
CGTGTAG282350.011.405761118-119
GCGTCGT401800.011.2786875118-119
AGCGTCG452000.011.229971118-119
GAGCGTC530850.010.452578118-119
AGAGCGT590900.010.276029118-119
AAGAGCG675650.09.902488118-119
ATCGGAA1010900.09.71592118-119
GATCGGA1085600.09.641762118-119
TCGGAAG958400.09.5221195118-119
AGATCGG1202000.09.405702118-119
CGGAAGA923600.09.323913118-119
TAGATCG362400.09.017644118-119
GTGTAGG339450.08.856435118-119
CAGATCG362000.08.3376118-119
AAGATCG381300.07.907782118-119
GAAGAGC1018650.07.531444118-119
GTAGGGA313600.07.528839118-119