FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28342680
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG120750.012.068364145
ACGTCCT1104500.011.670932145
TACGTCC1149050.011.660108145
CGTCCTG1066900.011.368724145
GGTGTAG641900.011.351106145
GTACGTC1238600.011.302921145
TGTACGT1355600.010.953121145
GTGTACG1461550.010.615479145
TAGTTCG26350.010.1803145
AGTGTAC1635600.09.977871145
GAGTGTA1789300.09.890634145
GTGTAGT261350.09.847939145
TGGTGTA745250.09.611572145
TAGATCT350200.09.564568145
AGAGTGT2068800.09.342887145
GATCTCG279300.09.266949145
AAGAGTG2237600.09.140293145
AAAGAGT2408300.09.061401145
CTGGTGT974350.08.83977145
TCGGTGG216900.08.623819145
Produced by FastQC (version 0.11.5)