FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19965483
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3047291.526279128834499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG348400.020.9249041
GCGTCGG148400.015.242829145
CTCTATG702900.013.192342145
GTGTAGT181100.013.091042145
CTATGTG572200.012.9746895145
TGTGTAG454600.012.519445145
GCCTCTA835950.012.324198145
TCTATGT631400.012.274902145
CCTCTAT752600.012.118847145
TATGTGT601650.011.7129755145
AGTGTGC1350550.011.638406145
ATGTGTA506050.011.504479145
ATCGGAC62800.011.429321145
GTGCCTC1091500.011.225555145
TGCCTCT1109750.010.8057575145
GTGTGCC1285700.010.781846145
TGTGCCT1272500.010.642999145
AGAGTGT1609100.010.19639145
GATCTCG178350.010.122112145
CGGTTTA25200.010.0749531
Produced by FastQC (version 0.11.5)