FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57009081
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGT91500.020.3154951
ATTCGTG92500.019.8926162
GTCGTAT58400.015.53132174-75
TATTACG63000.014.86665578-79
GCGTAAT64500.014.30049192-93
TACGTAG66700.014.1002480-81
CGTATTA65800.013.99983176-77
GGCGTAA70100.013.34012290-91
AGTCGTA71400.012.93302472-73
CGTGGAT145950.012.9182625
TTCGTGG160250.011.7664953
CGTAATC80250.011.69962592-93
ATTACGT82150.011.68754778-79
TCGTGGA160950.011.6270054
TTACGTA81950.011.41881480-81
ACGTAGC88250.010.73711682-83
GAGTCGT89850.010.67059472-73
TGGCGTA91700.010.60988190-91
TTGCACG92350.010.51545352-53
TCGTATT91550.010.34494474-75
Produced by FastQC (version 0.11.5)