FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36372533
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403420.11091336421359492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT576900.013.81156145
GCGTCGG237900.013.774936145
GTAGGCT1523700.012.790142145
GCTATAG844000.012.722081145
TATAGTG681500.012.425747145
TAGGCTA1255150.012.257314145
CTATAGT739050.012.007516145
AGGCTAT1205850.011.97081145
CGATAGT123500.011.858506145
AGTGTAG2415350.011.439431145
TGTAGGC1969450.011.408333145
GAGTGTA2513250.011.400577145
GGCTATA948500.011.351014145
ATCGGAC84650.011.134275145
TAGGCGA248900.011.127152145
GCGATAG153950.011.114191145
AGAGTGT2917550.010.215855145
AGGCGAT267550.010.107633145
TAGTGTT406050.010.016798145
AAGAGTG3156600.09.9130535145
Produced by FastQC (version 0.11.5)