FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40802486
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG192350.011.24123994
GATCGGA590000.010.60411894
CGTCGTG156450.010.42563294
ATCGGAA538900.010.2053194
GCGTCGT165400.010.20251994
AGAGCGT289950.09.92148894
AGATCGG676450.09.68669394
GAGCGTC252050.09.64167294
AAGAGCG339300.09.44819994
TAGATCG226050.09.39901794
TCGGAAG518900.09.36667394
GTCGTGT154600.09.09096994
CGGAAGA503100.09.05353194
CGTGTAG130550.09.03743894
TCGTGTA133000.08.97698794
CAGATCG259600.07.84028394
AAGATCG211700.07.482686594
GAGATCG275800.06.288985794
GAAGAGC685450.05.71924694
GTGTAGG221900.05.1263394
Produced by FastQC (version 0.11.5)