FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26484958
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT267000.011.248089118-119
TCGTGTA204850.010.857611118-119
AGCGTCG298200.010.849005118-119
CGTCGTG244650.010.574081118-119
CGTGTAG197600.010.247757118-119
GTCGTGT229800.010.209291118-119
AGAGCGT399500.010.040695118-119
GAGCGTC356000.09.9979925118-119
AAGAGCG457100.09.86181118-119
GATCGGA720500.09.702581118-119
AGATCGG804600.09.612336118-119
ATCGGAA668400.09.569134118-119
TCGGAAG648900.09.554257118-119
TAGATCG218100.09.489039118-119
CGGAAGA633950.09.024407118-119
CAGATCG284350.08.271645118-119
AAGATCG247700.07.598823118-119
GTGTAGG257300.07.0032797118-119
GAGATCG338450.06.9670243118-119
GAAGAGC756700.06.963196118-119
Produced by FastQC (version 0.11.5)