FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31490292
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG166150.018.021227145
CGTATGC184750.017.61962145
TATGCCG146050.016.97693145
CTCGTAT210450.016.811464145
TCGTATG179550.016.55515145
CGCGAAA632050.016.23079145
ATCGCGT62950.016.008614145
TCTCGTA262950.015.715795145
AATCGCG67950.015.577511145
CCGCGAA730200.015.558289145
GCGAAAT582750.015.538705145
CGAAATC543150.015.296761145
CGCGTAT46350.015.172482145
TCCGCGA816350.014.85779145
AATCTCG359150.014.110291145
ATCTCGT313450.014.08587145
CTCCGCG975250.013.9237585145
CACTCCG1172000.013.547249145
TCGCGTA55050.013.433138145
ACTCCGC1035300.013.375245145
Produced by FastQC (version 0.11.5)