FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31490292
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG197450.014.760932145
GCTATAG558750.012.949645145
GTAGGCT1013750.012.887503145
TAGGCTA818150.012.4505415145
CGATAGT90150.012.38511145
AGGCTAT810850.012.187095145
AGTGTAG1632650.012.029866145
ATCGGAC72950.011.826779145
TATAGTG462300.011.809076145
ATAGTGT398100.011.764818145
GAGTGTA1689850.011.609794145
TGTAGGC1327050.011.287243145
CTATAGT488050.011.200873145
GGCTATA625650.011.089843145
GCGATAG113000.010.843092145
TAGGCGA179150.010.603014145
AGAGTGT1989450.010.200342145
AGGCGAT197250.010.0711355145
AAGAGTG2172350.09.912234145
GTGTAGG4766450.09.775931145
Produced by FastQC (version 0.11.5)