FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76137428
Sequences flagged as poor quality0
Sequence length15-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC2677590.3516785463254682No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT2178020.28606429941394923No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG1838120.24142134141962346No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1708870.22444545933440252No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1365700.17937301480685688No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT1218220.16000277813429684No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC1082370.142160042495788No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG1065090.1398904622835434No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC889220.11679144191737079No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT846100.11112799870255664No Hit
GCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC810650.10647194438982098No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC802610.10541595915217941No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA798660.10489716043468136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)