FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105541451
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA3650340.34586789980744154No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC3543140.33571075311443277No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG3524100.3339067225823909No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3481440.32986470879578866No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG3262240.3090956177966513No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG3144380.2979284414045056No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG3123100.2959121719863412No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG2536510.24033306117801997No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2483000.23526301528676158No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2383530.22583828224988114No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG2234720.21173860874814013No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGGTT2158120.20448079683877TruSeq Adapter, Index 7 (97% over 35bp)
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG1907290.18071477906817862No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1760380.16679512962163084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1739390.16480633755925905No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1733270.16422647060253132No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1732150.16412035115947005No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1678130.15900198302181764No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC1513300.1433844224862893No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG1512350.14329441045869268No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC1507780.14286140523120153No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1495930.14173862362381204No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1462340.1385559878270008No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1429820.13547473399811416No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1401070.13275068579453206No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1393150.13200026973288437No Hit
GTGCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCG1376910.13046153780849573No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCGCGGTT1313260.12443073195952176TruSeq Adapter, Index 7 (97% over 35bp)
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1281650.12143570017812244No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1273870.12069854904685742No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1231100.11664611281495459No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1166850.11055845726434062No Hit
CCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAG1128810.10695418618036623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)