FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62024599
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA2770980.44675500441365207No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC2701540.43555944634160393No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG2177270.35103330535034977No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG1993870.3214643918939323No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG1984320.3199246802063162No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG1814870.29260487439830124No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1496710.2413090973792511No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1419350.22883662657778733No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG1412510.2277338383114738No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1410620.22742912050104508No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1409230.22720501586797845No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1334680.21518559112328967No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1313990.21184981784404602No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1274970.2055587654827079No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1219080.19654782451717262No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1174440.1893506800422845No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC1169180.18850262941643525No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG1102850.17780848530757934No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTAT1096620.1768040451176476TruSeq Adapter, Index 2 (97% over 38bp)
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1062630.17132396132057218No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1029580.1659954302969375No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG984320.1586983254821204No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCGCGTAT978500.1577599880976256TruSeq Adapter, Index 2 (97% over 38bp)
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG977250.15755845515422034No Hit
CACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGC930140.14996308158316346No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA910550.1468046572941165No Hit
GGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGAC895750.14441850724419839No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC851400.1372681184121803No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG828840.13363085184960244No Hit
GCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTG791500.127610659764201No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA778700.12554696242373126No Hit
GTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCG717330.11565250103430737No Hit
CTCCACACAAGCCGGAGGGTGCGCGGCGCCGGGCGACCGCGCCAACCCCG715980.11543484545542972No Hit
CTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCT713180.11498341166220195No Hit
GGCGGAAAGAGAAGCGCGACACCGGCGAGACAGTCAAACCACGCGGGAGG709920.11445781374580108No Hit
GTGCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCG684880.11042070582350721No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG679520.10955653256218553No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG644350.10388620166653557No Hit
CACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGT627340.10114374137267698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)