FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61751240
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1488780.24109313432410426No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1395150.22593068576436684No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1242400.20119434038895412No Hit
CACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGC1241410.20103401972170923No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1185490.19197833112339122No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG1038380.16815532773107067No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA946460.15326979668748353No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC942730.15266576023412648No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG924620.14973302560402024No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG887040.143647317851431No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG853070.1381462137440479No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA838710.1358207543686572No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG833610.1349948600222441No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG817490.13238438612730694No Hit
CTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCT800040.12955853194203062No Hit
GGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGAC780680.12642337222701924No Hit
CCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCA738780.1196380833809977No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG734340.11891906947941451No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG732530.11862595795647181No Hit
CACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGGGA729360.11811260794115228No Hit
CCGAGAAGCACACAGCACGCACCACGCGCGGCGCAGGCCCGCGTCGCGGG674770.10927229963317336No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA668900.10832171143445864No Hit
CCCGGGACGGGTCAAAAACCCGTAACGACGTACCGCCCCTCGTAGACACG652630.10568694652933286No Hit
GTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCG650960.10541650661589953No Hit
CAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGAGG646100.10462947788578819No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT639080.10349265860896072No Hit
CTCGACGCTTACAAGAAACAGCGCGTGCACACACCAGACGGGAAGGGTAT636070.10300521900450905No Hit
GGCGGAAAGAGAAGCGCGACACCGGCGAGACAGTCAAACCACGCGGGAGG619850.10037855110277949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)