FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95387267
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG4827240.506067544633604No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC4378480.4590214331227248No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG4119010.43181968930926595No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA3882450.4070197335667453No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG3735050.391566937335567No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTAT2983170.31274299954521184TruSeq Adapter, Index 6 (97% over 40bp)
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG2471680.25912053859347917No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA2412670.2529341783112415No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG2392340.25080286659224654No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA2360780.24749424889173102No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG2351750.24654758165992952No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG2254240.23632504325760795No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2123730.2226429236095002No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1996700.2093256325291299No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC1958010.2052695356079339No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1948350.20425682182507648No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCGCGTAT1896640.1988357628487249TruSeq Adapter, Index 6 (97% over 40bp)
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1645100.17246536689220796No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG1640930.17202820162569496No Hit
CCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAG1623000.17014849581548447No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1563760.16393802330032162No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1550520.16254999737019407No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC1542500.1617092142916727No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1481390.15530269883924863No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1458530.15290615255807674No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1448740.15187981012182683No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1412860.1481183017854993No Hit
GTGCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCG1408680.14768008816103306No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1359570.14253160225253125No Hit
GCCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAA1245390.13056145114211104No Hit
CTCCACACAAGCCGGAGGGTGCGCGGCGCCGGGCGACCGCGCCAACCCCG1171400.12280465064587708No Hit
GGCGGAAAGAGAAGCGCGACACCGGCGAGACAGTCAAACCACGCGGGAGG1106780.11603016155185576No Hit
GTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCG1093800.11466939292851319No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1092020.11448278521283141No Hit
CACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGGGA1084080.11365038899793618No Hit
CCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAA1029410.10791901606741704No Hit
GCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTG1027820.10775232715284734No Hit
CCCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACC972060.10190668320542194No Hit
CACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGC971780.10187732918273044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA954160.10003012246907128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)