FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99805955
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC5361270.5371693502657231No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG3960040.39677391995297273No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG3820130.3827557183336405No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA3797990.38053741382465606No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG3016900.3022765525363692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTAT2879620.28852186224759835TruSeq Adapter, Index 1 (97% over 37bp)
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG2865260.2870830703438487No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA2802810.2808259286732941No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG2676420.2681623556430075No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG2585990.25910177403743095No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA2519810.2524709071718216No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG2508510.25133871020020804No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC2467150.24719466889525782No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA2413800.24184929646733003No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG2341010.23455614447053783No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG2143320.21474870913263644No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCGCGTAT2039630.2043595494878036TruSeq Adapter, Index 1 (97% over 37bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1972860.19766956791305687No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1810930.1814450851154122No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1805170.1808679652431561No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG1803050.18065555306795072No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1721850.17251976597989568No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1617430.16205746440680818No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1575520.15785831616961132No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1545580.1548584951669467No Hit
CCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAG1539760.1542753636293546No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1535130.1538114634542598No Hit
GCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGATGCGCGGGGGAG1520860.15238168904851418No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC1423910.14266783980975886No Hit
CTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCT1325330.1327906736627088No Hit
GGCGGAAAGAGAAGCGCGACACCGGCGAGACAGTCAAACCACGCGGGAGG1306360.13088998547230976No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC1242790.12452062604881642No Hit
CACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGGGA1196900.11992270401099815No Hit
GTGCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCG1176840.11791280390032839No Hit
CAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGACCG1160760.11630167759028005No Hit
CTCCACACAAGCCGGAGGGTGCGCGGCGCCGGGCGACCGCGCCAACCCCG1106450.11086011851697626No Hit
CCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGC1064170.10662389834354073No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1052640.10546865665480583No Hit
GTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCG1045830.10478633263916967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1042760.10447873576281096No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCTCCGGCGAG1041050.10430740330073492No Hit
CACCTCTCTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGT1039040.10410601251197887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)