FastQCFastQC Report
Mon 13 May 2024
EGAF00005787782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67664539
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC2304020.34050627316030335No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG2174440.3213559173143853No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG2134640.31547395896689695No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1663880.24590132802057513No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG1625300.2401996709088641No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA1540260.22763178804779854No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1467970.2169482008885038No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG1409670.20833216642472063No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1397170.2064848176974944No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1285010.18990892703783885No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1282460.1895320678974847No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1125890.16639291667973974No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1122290.16586088024629858No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1108780.16386426574191246No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG1102420.16292433470949974No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1000220.14782041151569805No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC991310.14650362134293118No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTAT988400.14607355855923293TruSeq Adapter, Index 14 (97% over 38bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT929610.13738510802534248No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG851690.125869474999305No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG846950.12516896036194083No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG788590.11654405862426699No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG785570.11609773917176913No Hit
CCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAG775320.11458291321544362No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA752130.11115571185669351No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA715610.10575849781522934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)