FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108627848
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA5290960.487072154830868No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG4958390.4564566169072962No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG3934510.36220086031714444No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC3855040.3548850567305724No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG3602150.3316046544528803No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG3119960.28721548455972357No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG2780940.2560061762431306No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT2638600.2429027223295448No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC2561810.2358336326427087No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC2442430.2248438172134276No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG2270750.20903939844228525No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG2098840.19321380646332972No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1921710.17690767472444083No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1897020.1746347768944111No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1863780.17157478807828358No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1840310.16941420030708884No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1833330.16877163947867216No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1662480.15304362837050772No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1631080.15015302521688545No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG1517750.13972015721051567No Hit
GCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTG1511000.13909876958991216No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1510510.13905366145152762No Hit
GTGCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCG1440630.13262068857333895No Hit
GCCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAA1331050.1225330359117489No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1318530.12138047694731098No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1306800.12030064334883998No Hit
CCCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACC1238490.11401220062833242No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTAT1147020.10559170793846528TruSeq Adapter, Index 6 (97% over 37bp)
ACCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATC1111670.10233747795500836No Hit
GTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCG1107760.10197753342218471No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1097000.10098699552623007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)