FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99069790
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC4615140.46584735871550753No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG4180360.421961124576927No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA4123720.4162439427801351No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG3484310.351702572499649No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG3338210.33695539275898334No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG3042940.30715115071910415No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2263240.22844905596347787No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG2251970.22731147406288033No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG2174270.21946851810223886No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA2058670.2077999761582214No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1982430.2001043910560424No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1949360.19676634017292255No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1910880.1928822096019382No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1872030.18896073162161744No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG1819970.1837058501890435No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1718800.17349385720914517No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG1709140.172518787008633No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC1708420.1724461109688433No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC1594170.16091383659943156No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT1593550.1608512544540571TruSeq Adapter, Index 22 (97% over 38bp)
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1449070.14626759580291832No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1419910.14332421619143434No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1404770.141796000576967No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1337000.13495536833175886No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1278810.12908173117153068No Hit
CTCCACACAAGCCGGAGGGTGCGCGGCGCCGGGCGACCGCGCCAACCCCG1254420.12661983032365368No Hit
CCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAGAAG1217670.12291032412605296No Hit
CACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGC1201280.12125593483139513No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1178590.11896563018857717No Hit
GTGCATGCGGCCGGACCGACCCCGTCGGGGTCCGACAAAACCCGTCCGCG1078670.10887981088886936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCGCGTAT1063850.10738389573653079TruSeq Adapter, Index 22 (97% over 38bp)
GCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTG1030110.10397821576082882No Hit
GGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGAC997470.10068356862369446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)