FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97504097
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC3297640.338205275620367No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG3046270.31242482046677483No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA2857590.293073838733156No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG2752470.2822927532983563No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG2543540.26086493575752No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG2519340.258382988768154No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2465350.25284578554683707No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1992280.20432782429644983No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1829950.18767929310703732No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1818140.18646806195230956No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG1770340.18156570384934698No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTAT1640010.16819908603430275TruSeq Adapter, Index 7 (97% over 36bp)
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1570230.16104246368232097No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1568120.16082606251919854No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1508420.15470324288014278No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1493920.15321612588238215No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1483540.15215155523157145No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG1242700.12745105469773235No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG1183260.1213549006048433No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC1169990.11999393215240996No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG1138540.11676842666416365No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC1136990.11660945898509269No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1131260.11602179137149488No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG1105660.11339626067200026No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG1105430.113372671919622No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1002620.10282849960653448No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA990150.10154957898846034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)