FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64473532
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1747640.2710631705426034No Hit
CACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGC1650630.25601668604102534No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA1472040.2283169471776418No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1328750.20609232328081545No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC1235290.19159645232403277No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG1202970.18658354253028978No Hit
GGCACGGGACCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGAC1160400.17998083306495447No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1042120.16163532036681347No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA1000180.15513032541787847No Hit
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC995910.15446803814005414No Hit
CCGAGAAAGACTGGTGAGGCAGCGGTCCGGGAGGCGCCGACGCGAGGCCA992540.15394534302851595No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG932220.1445895658392036No Hit
CTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCGCTCT871710.13520431919256418No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG868590.13472039968277214No Hit
GGCGGAAAGAGAAGCGCGACACCGGCGAGACAGTCAAACCACGCGGGAGG839420.13019606247103077No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG836500.12974316344263565No Hit
CCGAGAAGCACACAGCACGCACCACGCGCGGCGCAGGCCCGCGTCGCGGG813660.1262006244671069No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG809410.12554143923742225No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG784350.12165457291838766No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA777950.12066191751368609No Hit
CACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGGGA773900.12003375276539835No Hit
GTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCG763010.11834468755333584No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG752820.11676419402616256No Hit
CTCGACGCTTACAAGAAACAGCGCGTGCACACACCAGACGGGAAGGGTAT737470.11438337207894862No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG735750.11411659593893507No Hit
CCCGGGACGGGTCAAAAACCCGTAACGACGTACCGCCCCTCGTAGACACG710930.11026695419757676No Hit
CTCCCCTGAGCAGTCCCACCACACCTGCAGCGCCGAGCCGACCCGAAACA679920.10545722855698365No Hit
CAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGAGG673840.10451420592251716No Hit
CTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGCA672250.10426759309541161No Hit
GTGAAGGAGAAGCGGAGACGAAGAAGAGCCACGGGGGCCACGGGCCCCGA658160.1020822001809983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)