FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005787810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59857935
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGAC2105530.35175453346327434No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG2071090.3460009103220818No Hit
CCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGACCGA1841480.3076417520918488No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG1673950.2796538169918491No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1569280.26216741356012363No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG1469500.24549794442457795No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG1387990.23188070219929907No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1379360.23043895516943577No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1375110.22972894069934086No Hit
CTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG1295600.2164458229305772No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1275610.2131062489877073No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1187570.19839809041190612No Hit
CCCAGACGCACCTCGGCCAACAGACCACCACACCGTCGGGACAATGACCA1165020.1946308371646967No Hit
GGAGAGAGGAAAAAAGACCGGCTGCGGCGAGCGGGGAGGGGGGAACGACA1092780.182562261795366No Hit
CCCACCACACCTGCAGCGCCGAGCCGACCCGAAACACGACGGCCCCCCGG1038360.17347073533358612No Hit
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC997840.1667013738445872No Hit
CCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGG981110.16390642276583714No Hit
GCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTG973720.1626718328990133No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG959140.16023606561101716No Hit
GTGGGACAGGACCAGGCGGGACAGAGAGCGCGAGAGAGGAGGAGGAGGCG934950.15619483030946524No Hit
GCACGGGCGAGGGCGGGAACGGCGGAGCGGGAAGAAGCCGCGAGCGCGGA926290.1547480714127542No Hit
CGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCGGG916170.15305740166278706No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA856100.14302197361135163No Hit
CTCCCTCGCAACGCCACCGGGGATACCACCTCTCTCCGTTCTCGCCCTCG844310.14105230994019424No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCTCCGGCGAG807880.134966232964769No Hit
CTCCCCCAGCGCAAGACCCAAACACACACAGACAACCACCGCAGGGCGAC740330.12368117944596652No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG716160.11964328538897975No Hit
GGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGA713840.11925570101942208No Hit
GCCCCGGCCACCGAGAGAGGATGCATGCGACGAGCACACAGGGAAACCAG696830.11641397251675989No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG684120.11429061159560551No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC630850.10539120669632188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)