FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156359227
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2192990.14025331552707151No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1783490.11406362350461095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT586600.051.1008266
TCGACCG840750.047.471558145
AGACCGC630600.047.3405724
CCGCGTT649000.046.197117
CAGACCG700000.043.2596473
CTCGCTA549750.034.7592351
GCGTTCT863500.034.7288749
TCGCTAT587750.032.4803542
GTGCTCT1569750.032.11082145
CGCTATG615000.030.9207153
CGCGTTC989150.030.2188668
CTTCGAC1153400.028.270956145
GACCGCG1093500.027.525525
ACCGCTA249050.027.3348982
CCGCTAA313350.023.65693
CGCTAAG345700.020.6538124
CGATCCT1134850.020.018454145
TCAGACC1647350.019.569422
CGGTGCT990350.019.463654145
TCGTACG208100.018.29016910-14
Produced by FastQC (version 0.11.5)