FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156359227
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1706180.10911923988982115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1054900.030.3502394
CAATCGC442200.028.4295561
TCAATCG307900.024.5151423
GCGACAT543150.023.4057947
TGCGACA555850.022.8118826
GGCGATC450550.019.9532052
GGGCGAT457750.019.9514681
AATCGCC632950.019.9050662
GGGCGAG1694150.019.6666975
GCGAGGC1648950.019.578297
GGCGAGG1764850.018.8308776
TACCGTA233300.017.8090749
CGACATC745050.017.447718
TCGCGTC275350.017.407179
CGGAGGG2028050.016.98521
CTCGCGT276850.016.8670048
GAGGGCG2078500.016.1556193
TGGCGCG1199700.015.9872654
GGTCACG821250.015.840874145
CTGCGAC822100.015.7566155
Produced by FastQC (version 0.11.5)