FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126415618
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2169290.1715998414056719No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1682670.13310617996583302No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1613120.12760448633807256No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1522630.12044635181073908No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1467630.1160956235644871No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1444630.11427622811605445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT407200.051.7709966
CCGCGTT448100.046.9014137
AGACCGC465350.045.221014
TCGACCG460950.042.78672145
CAGACCG528300.040.6037063
CTCGCTA634300.037.557231
GCGTTCT587100.035.8852429
TCGCTAT670000.035.303972
CGCTATG714300.032.9984933
CGCGTTC654550.032.118238
GACCGCG766600.027.6091465
CGATCCT1691550.024.640366145
CTTCGAC674500.024.065199145
ACCGCTA186100.021.571542
TCGCGTC812950.021.448089
CTCGCGT834200.020.7932648
GCTATGT1212650.019.7091084
GTCGGCT334000.019.645351
TCAGACC1203600.018.7984392
GTCGCGT70550.018.5266441
Produced by FastQC (version 0.11.5)