FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126415618
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG224600.028.7187443
TGGCGCG1303650.027.0174124
CGGTGGC1931600.024.8541431
GTGGCGC1627050.022.5498543
CAATCGC291900.022.0759641
GGCGCGT1557800.021.0844275
AGGGCGA750050.020.6589894
CGTGCCT1818050.018.6477629
GCGACAT347400.018.247767
TCGCGTC207550.018.1635049
GGGGACC5084800.018.15052145
TGCGACA342850.018.0921846
GGTGGCG2399000.017.9246062
CTCGCGT208750.017.7483188
GGTCACG630600.017.697805145
GCGTGCC1916050.016.9320968
ACGCCCG1196900.015.8618456
AATCGCC438400.015.3186735
CGCGTGC2166700.014.9882227
GGCGATC305800.014.9774912
Produced by FastQC (version 0.11.5)