FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129710534
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1357420.10464994308018191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1885600.025.8717781
TCAATCG225750.025.1286563
TGGCGCG1427100.024.3929794
GCGACAT378650.023.8260827
TGCGACA373450.023.6591646
AGGGCGA706300.022.2585244
CAATCGC298950.021.5566841
GTGGCGC1736900.020.9632843
TACCGTA190300.018.9835559
GGCGCGT1783850.018.4422385
GGTGGCG2367550.017.6008242
CGACATC547550.016.769668
GGGGACC5836400.016.307878145
GGTCACG640400.016.10147145
CGTGCCT2191000.015.6945679
GCTGCGA588200.015.5674864
CTACCGT238300.015.4021178
GCGTGCC2118600.015.3525948
CGGAGGG1066550.015.3034051
CTGCGAC623150.014.7866735
Produced by FastQC (version 0.11.5)