FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118749709
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1968390.16575956409291076No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1689870.14230519082787815No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1548690.13041631958862315No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1285410.108245317889579No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1258110.10594636488751312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT487800.057.2161146
CCGCGTT534550.052.164147
AGACCGC541350.051.4258044
CAGACCG606900.046.435743
TCGACCG745450.043.164085145
GTGCTCT1192950.039.915356145
GCGTTCT706500.039.4957779
CTCGCTA615000.035.9392361
CGCGTTC809400.034.3554848
TCGCTAT662600.033.1679462
GACCGCG896600.031.2648095
CGCTATG719700.030.4540653
ACCGCTA195250.028.4355282
CTTCGAC999050.026.666428145
CGATCCT1266050.025.107918145
TCAGACC1319000.023.2264522
CCGCTAA268400.022.3820253
CTCAGAC1581250.021.722541
AACGCTA553300.019.613146115-119
CGCTAAG328850.019.105334
Produced by FastQC (version 0.11.5)