FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118749709
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT290350.024.7246461
TCAATCG180150.023.5425873
GGCGATC313100.022.6062932
TGCGACA443400.022.2881156
TGGCGCG1094450.021.2093184
CAATCGC252850.020.8654441
GCGACAT493000.020.5171057
CGGTGGC1661000.020.4523451
AGGGCGA715950.018.5642154
GTGGCGC1442800.018.1295243
CGACATC609800.016.7913678
GGCGCGT1260300.016.5644115
TACCGTA166600.015.8102069
CGGAGGG875900.015.3205151
CGGCCGC2551100.015.312589145
CTGCGAC661000.015.2737335
GCTGCGA662050.015.1030134
GGGGACC3828200.015.072896145
ATATCGG237500.014.905279145
CGATCTG508250.014.7708434
Produced by FastQC (version 0.11.5)