FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125393258
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1672520.13338197178033287No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1620090.1292007262463824No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1394680.11122448066545969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT416750.050.1427766
CCGCGTT451450.046.2201167
AGACCGC474350.043.7285544
CAGACCG509500.041.3188443
TCGACCG451900.039.798443145
CTCGCTA487600.035.0131531
GCGTTCT631600.033.0076339
TCGCTAT517000.032.882032
CGCGTTC639950.032.4899678
CGCTATG555600.030.586293
GTGCTCT1240950.030.577423145
GACCGCG729950.028.6538165
ACCGCTA174100.024.91782
CGATCCT1107100.023.682117145
CTTCGAC687250.021.903631145
CCGCTAA222850.020.875443
CGTATCG122700.019.713453
CCGGTAA161050.019.4714491
CGGTGCT771350.018.976383145
TCAGACC1217700.018.844732
Produced by FastQC (version 0.11.5)