FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125393258
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1520150.1212306007712153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC271050.022.6885871
TCAATCG204300.021.7204633
AGGGCGA711700.021.0487424
TGGCGCG843950.019.7503644
CGACCGT94600.018.8968561
CGGAGGG825050.018.88241
TGCGACA306250.018.8038946
CGGTGGC1521350.018.395121
GCGACAT315550.018.3873527
GGGGACC3244650.018.185675145
TACCGTA188750.018.1806819
AATCGCC359050.016.8788952
GGCGATC241500.015.9412982
CTACCGT228350.015.544158
GTGGCGC1150050.015.4394763
GGGCGAT256000.015.1461781
GGCGCGT1026050.015.083565
GGGCGAG1038650.014.7870635
GCGAGGC1016250.014.7221917
GGCGAGG1084950.014.3333386
Produced by FastQC (version 0.11.5)