FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158720064
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3109220.19589331818817818No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2353490.1482793000889919No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2071450.13050964999610887No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1928800.12152212841849659No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1619220.10201734797687582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT631000.063.541116
CCGCGTT685950.058.468917
AGACCGC697950.057.216794
TCGACCG733250.055.656754145
CAGACCG751150.053.90143
GTGCTCT1568200.049.317856145
GCGTTCT896700.044.754519
CTCGCTA797000.039.900321
CGCGTTC1031850.038.7614258
TCGCTAT886050.035.8984532
GACCGCG1149700.034.9969445
CGCTATG972700.032.584143
ACCGCTA182050.026.9763762
CGATCCT1614750.026.637833145
TCAGACC1692550.025.5206952
CTCAGAC2072900.023.7700041
CTTCGAC1025300.022.79736145
ACGCTAC722900.020.362852115-119
AACGCTA759300.019.995619115-119
GCTATGT1654850.019.4348344
Produced by FastQC (version 0.11.5)