FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158720064
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG228300.026.4325373
GCGACAT444250.024.6924637
TGCGACA453600.023.7202456
CAATCGC317800.023.6960541
CGGTGGC2046050.018.344711
TACCGTA201950.018.3298449
AGGGCGA948200.018.061754
TGGCGCG1343800.017.7503784
CGACATC631700.017.5022138
CGACCGT112300.016.5973971
CGGAGGG1144650.015.3217631
GGGCGAT336450.015.2996931
GCTGCGA722500.015.2664144
GGCGATC340600.015.2555742
CGGCCGC3383100.015.162058145
CTGCGAC762050.014.6787335
AATCGCC519650.014.6177792
GGGGACC5037000.014.408193145
GTGGCGC1815450.014.3721943
ACGCCCG995800.014.1996416
Produced by FastQC (version 0.11.5)