FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138684372
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1710150.12331238014330845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG206700.031.982523
CAATCGC296550.030.9608251
CGGTGGC2069350.029.7363871
TGGCGCG1435250.027.885654
AGGGCGA852600.027.5999374
GCGACAT438450.025.2712487
TGCGACA434950.025.110066
CGGAGGG990250.024.2308251
GTGGCGC1815300.022.6378123
GGGCGAT320450.022.2958551
GGCGATC317450.022.1622312
AATCGCC439150.020.649282
GGGGACC5951100.020.6101145
GGCGCGT1862750.020.5371915
CGGCCGC3066150.020.337505145
GGTGGCG2558500.019.587792
CGTGCCT2115950.018.7918979
GCGAGGC1247150.018.7829997
GGGCGAG1309450.018.4592385
GGCGAGG1343600.018.1540896
Produced by FastQC (version 0.11.5)