FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118476882
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG1226750.029.2374484
TGCGACA491350.028.1376276
CGGTGGC1726450.028.0646021
GCGACAT502000.027.9647667
GGGCGAT319000.024.7756941
CTCGCGT230300.024.3491698
GTGGCGC1536150.024.3369563
TCGCGTC231500.024.1394259
CGACATC623400.022.6431148
GGCGATC359150.022.5407852
GGCGCGT1509250.022.3535165
CAATCGC260250.021.659451
AGGGCGA722100.020.8724944
GCTGCGA675350.020.702794
CGTGCCT1701100.020.3206129
CTGCGAC691500.020.2926865
GGTGGCG2146950.019.9210112
TCAATCG183850.019.2677123
GCGTGCC1720800.019.2526978
CGATCTG440400.018.6793484
Produced by FastQC (version 0.11.5)