FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114948120
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1290460.11226455900279186No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1261720.10976430062536038No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1167540.1015710391783702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC308850.031.3701551
TGGCGCG1280200.030.4879044
AGGGCGA779150.030.2962724
TCAATCG206750.028.7323573
CGGTGGC1932000.028.3795191
GCGACAT409150.026.366747
GGCGATC281200.026.2663022
GGGCGAT284000.026.0728031
GTGGCGC1587100.025.901943
CGGAGGG963250.025.4603271
TGCGACA434150.024.8033666
AATCGCC465050.020.7294622
GGGCGAG1196150.020.4815045
CGTGCCT1836500.020.3831169
GGCGCGT1826100.020.3692785
GAGGGCG1193850.020.0447793
CGACATC558700.019.5880188
GCGAGGC1236400.019.2250447
GGTGGCG2591300.018.9646592
GCGTGCC1920600.018.9261678
Produced by FastQC (version 0.11.5)