FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154663553
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2534400.1638653678155189No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2052280.13269318854972897No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2048870.1324727099732411No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1740580.11253976559041029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG783500.056.574764145
ACCGCGT420450.049.1561166
CCGCGTT462700.044.56997
AGACCGC495550.041.459174
CTCGCTA830450.040.7991031
CAGACCG539250.039.069233
TCGCTAT908600.037.165972
ACCGCTA222450.034.6186032
CGCTATG987800.034.1396373
GCGTTCT653900.031.5475589
CGCGTTC709750.029.0196068
CTTCGAC1097500.026.657986145
GACCGCG809900.025.678365
GTGCTCT1407900.025.600313145
CGATCCT1909000.024.022882145
CCGCTAA354050.023.6893123
CGCTAAG395550.020.6489074
GCTATGT1665200.020.5812844
GTCGCGT80450.020.2710421
TCGTACG250550.018.77652710-14
Produced by FastQC (version 0.11.5)