FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154663553
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG239600.025.6198963
TGGCGCG1514450.025.1724784
CGGTGGC2214100.025.1281091
GCGACAT513450.024.5951567
CAATCGC341400.023.7301121
TGCGACA542050.023.2477826
GTGGCGC1921700.021.2493293
GGCGCGT1697950.019.5017575
CGACATC702650.018.3880048
AGGGCGA910900.018.2507824
TACCGTA185000.018.2403859
GGTGGCG2705850.017.7124732
GGGCGAT378000.017.2048571
CGTGCCT2107000.016.4559549
GCTGCGA786650.016.3079054
GGGGACC5866650.016.227167145
GGCGATC407050.016.0529672
CTGCGAC817250.015.8719735
GCGTGCC2108000.015.51845558
AATCGCC524000.015.5041042
Produced by FastQC (version 0.11.5)