FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141592197
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2477060.1749432562304263No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2048710.144690882930505No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1836770.1297225439619388No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1610400.1137350810369868No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1524910.10769731894194708No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1439040.10163271920980221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT558750.055.0465556
AGACCGC589650.051.6283074
CCGCGTT602950.050.9911967
GTGCTCT1254750.049.035526145
CAGACCG644300.047.9358373
TCGACCG500900.041.881157145
CTCGCTA834000.040.3005871
GCGTTCT768550.039.7510839
ACCGCTA277550.038.9776842
TCGCTAT931900.036.088132
CGCTATG1031100.032.7454833
CCGCTAA387700.030.217263
CGATCCT1737650.026.395552145
GTCGCGT116600.025.4063641
CGCTAAG455050.025.3453714
CGCGTTC1286850.023.6888778
TCAGACC1486050.022.0231442
GCTATGT1570000.021.7604264
CACCGCT557000.020.753071
CTCAGAC1811900.020.6141071
Produced by FastQC (version 0.11.5)