FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99194488
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1522280.15346417232376863No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1255740.1265937276676099No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1140960.11502252020293709No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1048350.10568631595739472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT420000.050.3678256
CCGCGTT450350.047.0021137
AGACCGC454600.046.2367134
TCGACCG557600.043.575302145
CAGACCG504000.042.4588053
GTGCTCT1013700.037.77126145
GCGTTCT562900.037.5810059
CTCGCTA372550.037.1220441
TCGCTAT392300.034.9062922
ACCGCTA173650.032.916372
CGCGTTC644600.032.6479038
CGCTATG420750.032.503083
GACCGCG706650.030.0001095
CTTCGAC751850.027.724022145
CGGTGCT654800.026.99424145
CCGCTAA234050.026.834573
CGATCCT923250.024.85216145
CGCTAAG248250.024.0800884
TCGCGTC496250.023.0665879
CTCGCGT504050.022.7096428
Produced by FastQC (version 0.11.5)