FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99194488
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC228050.024.7034661
TGCGACA333600.024.0364286
GCGACAT361900.022.4969227
AGGGCGA567350.021.371264
TACCGTA144250.020.340389
TCAATCG163600.019.7557073
CGGTGGC1426750.019.0174261
GGCGATC268050.018.5340442
GGGCGAT272300.018.2352221
TGGCGCG782050.017.847944
CTCGCGT171800.017.8325988
TCGCGTC173700.017.600259
CGACATC477150.017.3209768
CTGCGAC485750.016.7475855
AATCGCC344450.016.4728432
CGTTCTT505450.016.425034145
GCTGCGA501450.016.3265634
CTACCGT186300.016.027468
CGACCGT89500.015.9559611
GGGGACC2951750.015.682439145
Produced by FastQC (version 0.11.5)