FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115921853
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1447360.12485652726755497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1594600.025.7122081
CAATCGC266000.025.510061
TCAATCG180750.024.485923
TGGCGCG1029900.022.665664
TACCGTA191250.022.067769
TGCGACA377450.021.6445776
GTGGCGC1340200.020.7754673
GCGACAT406350.020.5134727
CTACCGT214850.019.328768
GGGGACC4150750.019.022886145
GGCGATC247450.018.9753362
GGGCGAT250950.018.4239161
GGCGCGT1202500.017.9006715
AGGGCGA667350.017.5587624
GGTGGCG2058650.016.88122
CGGAGGG756800.016.3187831
AATCGCC421850.016.0515082
CGACATC547050.015.5311018
ACTACCG269750.015.5077077
CGGCCGC2455750.015.251022145
Produced by FastQC (version 0.11.5)