FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119921321
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1464740.12214174992285148No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1461090.12183738369593176No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1280960.10681670192742457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA572150.038.6778951
TCGACCG480800.037.798523145
TCGCTAT597350.036.857532
ACCGCGT322300.036.5737576
CGCTATG616200.035.479153
CCGCGTT373500.031.5763387
CTTCGAC762400.030.367434145
AGACCGC392150.030.0091724
CAGACCG430800.027.9402713
CGGTGCT649000.023.640305145
GCGTTCT536750.022.1065949
CGATCCT1189900.022.05515145
GTCTCGC680550.021.7616314
CGCGTTC556150.021.135518
GACCGCG595700.019.9088125
GTGCTCT1078900.019.845442145
CGTATCG176400.019.209663
CCCGTAT283000.018.1243551
GCTATGT1240450.018.0088734
GTATCGA171850.017.9666674
Produced by FastQC (version 0.11.5)