FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119921321
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA717350.029.2990234
CAATCGC292500.025.7599831
TGGCGCG934450.025.0534974
CGGTGGC1596100.023.8068561
GCGAGGC955450.021.8434437
GTGGCGC1200950.021.5631623
GCGACAT345700.021.05317
GGGCGAG1040300.020.7488355
CTACCGT234250.020.6578258
TGCGACA345500.020.6477786
GGCGATC314100.020.4250872
GGCGAGG1055850.020.3190566
CGACCGT91250.019.7914451
ACTACCG268550.018.3605277
GGGGACC3997250.017.775293145
GGGCGAT361650.017.6517031
CGGAGGG1333300.017.2861391
GGTGGCG1871200.017.2409532
TACCGTA280900.017.1570849
GAGGGCG1334300.016.764033
Produced by FastQC (version 0.11.5)