FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114541890
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2114830.18463376149983207No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1918350.16748021182468703No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1869320.16319968179327232No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1714540.14968672160028093No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1650090.1440599591992065No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1149870.10038860018810586No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1147470.10017906985819772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG465450.047.076538145
ACCGCGT302250.043.1002966
CTCGCTA591800.042.1603551
CCGCGTT333350.039.2487377
TCGCTAT631650.039.206882
AGACCGC334700.038.772094
CGCTATG676550.036.7661443
CAGACCG402150.033.160013
ACCGCTA158450.031.679382
GCGTTCT490150.026.7826489
CGCGTTC494750.026.2798448
CGATCCT1519200.025.060207145
GACCGCG540000.024.3567375
CTTCGAC695100.023.801537145
CCGCTAA235350.023.513353
GTGCTCT1042750.023.301128145
CGTATCG114050.021.69973
GTCTCGC615550.021.2350544
GCTATGT1219450.020.7271044
CCCGTAT206300.020.5556181
Produced by FastQC (version 0.11.5)