FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114541890
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1757540.15344080667780147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1478150.031.6276281
TGGCGCG1036800.027.9343554
TACCGTA184750.025.4073149
GGCGCGT1103400.023.7680635
GTGGCGC1314300.023.6623363
CAATCGC234400.022.593821
GGGGACC4023050.021.830757145
GGTGGCG1791750.021.758062
TCAATCG165300.021.4524573
CTACCGT228850.020.6766728
TGCGACA319550.020.6509026
AGGGCGA615550.020.3317584
GCGACAT335650.020.0733997
CGTGCCT1406550.019.5031459
GCGTGCC1405650.018.4069948
GGGCGAT236600.018.0095921
ACTACCG266950.017.9369327
CGGAGGG731300.017.7683931
ATATCGG216350.016.896511145
GGCGATC257650.016.7685052
Produced by FastQC (version 0.11.5)